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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Bioengineered AAV9 and Optimised Microdystrophin Vectors Augment Phenotypic Rescue in a Murine Model of Duchenne Muscular Dystrophy
doi: 10.1111/jcmm.71078
Figure Lengend Snippet: Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained in ZOE‐Fluorescent cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Article Snippet: The images were obtained in the
Techniques: Biomarker Discovery, In Vitro, Transduction, Microscopy, Comparison, Control, Software